RNA
Part:BBa_K1941004
Designed by: Dimitri Coukos Group: iGEM16_EPFL (2016-10-13)
scCyc1_2PP7
Scaffold RNA with two PP7 hairpins that targets the region c6 of CYC1 promoter
Usage and Biology
A scaffold RNA is a guide RNA (gRNA), supplemented with one or more additional hairpins that can recruit different RNA binding modules. In this case, the hairpins are two times PP7, PP7 being a well-characterized viral RNA sequence which is recognized by PCP RNA binding module. This allows the recruitment of fusion proteins, like BBa_K1941000, to act on the level of expression of the promoter CYC1.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 231
Illegal NgoMIV site found at 260 - 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
None |